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John Doench
johndoench.bsky.social
did:plc:tucx4kdithgkxegzeqymsjnn
The motivation for this work was fatigue from bespoke cloning campaigns every time we wanted to try some new CRISPR thing (new Cas protein, domain, etc.).
You know, figure out what starting vector to use, what strategy to make the new thing. Esp. annoying b/c all CRISPR vectors kinda look alike.
2023-10-28T09:55:14.414Z